Ultrasonic Sonicator Cell Disruption Instruments Herbal Extract

Ultrasonic cell crusher simply means that the electric energy is converted into sound energy through transducer. This energy is transformed into dense small bubbles through liquid medium. These small bubbles explode rapidly and produce a large amount of energy, which plays the role of breaking cells and other substances.

 

Principle of ultrasonic cell pulverizer

Ultrasound is a kind of elastic mechanical wave in the material medium. It is a wave form. Therefore, it can be used to detect the physiological and pathological information of human body. At the same time, it is also a form of energy. When a certain dose of ultrasound propagates in the organism, through the interaction between them, the function and structure of organism can be changed, that is, ultrasonic biological effect.

 

The main effects of ultrasonic cell grinder on cells are thermal effect, cavitation effect and mechanical effect.

 

The thermal effect is that when ultrasound propagates in the medium, friction hinders the molecular vibration caused by ultrasound, so that part of the energy is converted into local high temperature (42-43 ℃). Because the critical lethal temperature of normal tissues is 45.7 ℃, and tumor tissues are more sensitive than normal tissues, the metabolism of tumor cells is hindered at this temperature, and the synthesis of DNA, RNA and protein is affected, thus killing The cancer cells were injured but the normal tissues were not affected.

 

Cavitation effect is the formation of vacuoles in organism under ultrasonic irradiation. With the vibration of vacuoles and their violent explosion, mechanical shear pressure and turbulence are produced, resulting in tumor bleeding, tissue disintegration and necrosis. In addition, the instantaneous high temperature (about 5000 ℃) and high pressure (up to 500 × 104pa) will be generated when the cavitation bubble breaks, which can cause the thermal dissociation of water vapor to produce · OH radical and · H atom. The redox reaction caused by · OH radical and · H atom can lead to polymer degradation, enzyme inactivation, lipid peroxidation and cell killing.

 

Mechanical effect is the primary effect of ultrasound. In the process of ultrasonic propagation, the medium particles compress and stretch alternately, which results in the change of pressure, which leads to the damage of cell structure. The killing effect is closely related to the frequency and intensity of ultrasound.

Type	Power (w)	Output Amplitude	Available Converter
PU-100UP	100	1/8″3/16″1/4″	100ul～80ml    1%～99%
PU-150UP	150	1/8″3/16″1/4″	100ul～100ml  1%～99%
PU-250UP	250	1/8″3/16″1/4″	100ul～200ml   1%～99%
PU-500UP	500	1/8″3/16″1/4″1/2″	250ul～500ml   1%～99%
PU-800UP	800	1/8″3/16″1/4″1/2″3/4″	250ul～800ml   1%～99%